Chemically modified primers for improved multiplex polymerase chain reaction

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Rfbo157 by Multiplex Polymerase Chain Reaction

A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for virulence factor and rfbO157 encoding the O-antigen specific for E. coli O157: H7. Multiplex PCR generated two products of 215 bp and 420 bp for vt and rfbO157, respectively. Multiplex PCR detected...

NEW PRIMERS FOR DETECTION OF Leishmania infantumUSING POLYMERASE CHAIN REACTION

Leishmania infantum causes visceral leishmaniasis (VL) in the New World. The diagnosis of VL is confirmed by parasitological and serological tests, which are not always sensitive or specific. Our aim was to design new primers to perform a Polymerase Chain Reaction (PCR) for detecting L. infantum. Sequences of the minicircle kinetoplast DNA (kDNA) were obtained from GenBank, and the FLC2/RLC2 pr...

Species-specific Polymerase Chain Reaction Primers for Detecting Prevotella nigrescens

A previous study reported the cloning of a putative Prevotella nigrescens-specific DNA probe, Pn23, using random shotgun method. The present study evaluated the species-specificity of Pn23 for P. nigrescens using the clinical strains of Prevotella intermedia and P. nigrescens to develop Prevotella nigrescens-specific polymerase chain reaction (PCR) primers. The specificity was tested against 10...

A Diagnostic Multiplex Polymerase Chain Reaction Assay for Ocular Herpetic Infections.